They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. FOR HTRF CERTIFIED READER. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. a one- plate assay protocol. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. Plate 25 μL of cells in. HTRF Signal + 5 hours @ RT or . Revvity. acceptor reagent. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Incubate 4h at RT. HTRF is compatible with a large range of available microplate readers. The VICTOR Nivo is a HTRF certified microplate reader. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. The emission of light by the acceptor will be proportional to the level of interaction. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. * For HTRF microplate recommendations, please visit . support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. + 4 µL HTRF pre-mixed antibodies . 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Alpha Technology: The VICTOR Nivo system. Read & analyse. 5 μL of Binding buffer 2X. culture plate. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. To perform the assay, use white plate only. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). As shown in Fig. It accepts all types of microtitration plate. Plate cells Stimulate cells 3. Endpoint mode experiments measure each well of the microplate only once. For more information about HTRF. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. 1. In addition, stacker. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. In particular, these parameters should be entered as below. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Even if not listed, some. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more. 96-well culture plateFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. As shown in Fig. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Due to the chemical and photophysical. 5 nm filter (PerkinElmer 2100-5110) were used. An. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". The kit includes everything you need: reagents, buffers and a microplate. For more ® information about HTRF compatible readers and for set-up recommendations, please. Combined with our Kaleido™ workflow software, it. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . 25,000 human Jurkat cells were plated in 96 well plates. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. VICTOR Nivo Multimode Plate Reader. Download the brochure to learn how this compact and robust device can handle various detection modes, plate formats, and software options. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. It measures at any wavelength, with top and bottom reading for most standard technologies. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. Read & analyse. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). PURCHASE SEPARATELY . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. If not, our scientific team will help you choose the instrument that best suits your needs and budget. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. They can be used in a no-transfer. FOR HTRF CERTIFIED READER For more information about HTRF. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. FOR HTRF CERTIFIED READER. Optimized instrument settings for the HTRF camps HiRange assay. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. -CERTIFIED READER IS NEEDED. Lyse cells Read on an HTRF compatible reader . For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The dual measurement. + 4 µL HTRF pre-mixed antibodies. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. The FLUOstar ® Omega is a multi-mode microplate reader with three detection modes. 0. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. For more information about HTRF. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. No washing steps are required. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. Provide more sensitive and precise results. Your lab might even have one already. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER. For more information about HTRF. Plate 25 μL of cells in. This protocol gives the cells viability and confluence to be monitored. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. For more information about HTRF ®Highly compatible plates for HTRF. • HTRF®-Certified Reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. It features a modular design that allows users to add or change detection cartridges according to their needs. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Certified for use with Alpha, LANCE ® or DELFIA ®. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Make sure the setup for Tb Cryptate is used. Revvity. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. best fluorescence polarization plate reader on the market. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more ® information about HTRF compatible readers and for set-up recommendations, please. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. culture plate. Choosing the right microplate reader ensures you’ll get an optimal readout. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. use of a wide variety of plates or sample matrix formats. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . For more information about HTRF. If not, our scientific team will help you choose the instrument that best suits your needs and budget. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. FOR HTRF CERTIFIED READER . 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The plate is set up for reading and images are acquired according to specified parameters. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. Microplate Readers and Reader Accessories. Specialized filters allow for IR fluorescence. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. For more information. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Unlike standard ELISA plate readers, MSD. No washing steps are required. The plate reader. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. Push the screw down and turn it clockwise to lock the plate. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Customer Transformation Hub. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. Its. VICTOR® Nivo™ multimode plate reader. For more. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. For more information about HTRF ®Excitation range. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. CLARIOstar is equipped with a specific optical device which enables the measurement of. For more information about HTRF. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Not for use in diagnostic procedures. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Make sure the setup for Eu3+ Cryptate is used. For more information about HTRF. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The laser beam passes through the sample well. For more information about HTRF. Smallest and most affordable multimode plate reader in the industry. FOR HTRF CERTIFIED READER. For more ® information about HTRF compatible readers and for set-up recommendations. 3-in-1 absorbance using a variety of sample formats, including microplates. In this case the Z´(pronounced Z prime) value comes to mind. HTRF-compatible SpectraMax Multi-Mode Readers. Excitation lasers significantly improve performance and lower. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. MATERIALS PROVIDED: KIT . FOR HTRF CERTIFIED READER. Microplate readers EnVision ® Multimode Plate Reader VICTOR® Nivo™ EnSight® Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ® The EnVision ® multimode plate reader provides exceptional speed,. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Use simpler, faster protocols. Make sure the setup for Eu 3+ Cryptate is used. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. * For HTRF microplate recommendations, please visit . Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. For the standard curve described, all wells of the microplate are read. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. 5% and an increased Z’ value of 0. But sometimes these parameters are not sufficient enough. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. After defining the objectives and their locations in Gen5: 1. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. HTRF-specific. 2 Locking the Plate Carrier Upon closing the software or pushing the plate carrier in/out button for more than three seconds (figure 10), the plate carrier will move into the respective lock position. FOR HTRF CERTIFIED READER. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. For more information. Incubate overnight at RT. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Highly compatible plates for HTRF. FOR HTRF CERTIFIED. Remove the plate sealer and read on an HTRF® compatible reader . Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. No washing steps are required. + 4 µL HTRF ® pre-mixed antibo dies 2. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Incubate overnight at RT. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Incubate overnight at RT. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Incubate 2h at RT Transfer 16 µL supernatant. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Conclusion. For more information about HTRF ®• HTRF®-Certified Reader. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. 5 EXAMPLE OF MAP PLATE . The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. 2. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. Make sure to use the set-up for Eu Cryptate. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. FOR HTRF CERTIFIED READER. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED READER. Incubate 24 hours at RT. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. This protocol gives the cells viability and confluence to be monitored. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. overnight at 4 C [Prostaglandin E2] pg/ml. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. (665 nm and 620 nm) on a compatible HTRF ® reader. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. The VICTOR Nivo is a HTRF certified microplate reader. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. Incubate overnight at RT. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. 47 seconds for a 1536-well plate. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. CLARIOstar is equipped with a specific optical device which enables the measurement of. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Handle complex biological matrices easily. The mean and standard deviation. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. + 4 µL HTRF pre-mixed antibodies. Step 1 Step 2 Step 3 Step 4 Step 5. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. See the Cytation 5 Operator’s Manual for more information and instructions. Read & analyse. The sample is then added and incubated 1. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. Plate 25 μL of cells in. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. 1 – 1. 96-well culture plateFOR HTRF CERTIFIED READER. Transcreener® ADP2 TR-FRET assay. FOR HTRF CERTIFIED READER. $352. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Make sure the setup for Tb Cryptate is used. Read & analyse. Read & analyse. 14 seconds for a 384-well plate. At Cisbio we provide microplates that help you discover better. FOR HTRF CERTIFIED READER. The VICTOR Nivo. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. For instance, when assay volumes are. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. Bench top solutions for cell based assays. 4. Configure your microplate reader. It can also be further streamlinedFOR HTRF CERTIFIED READER. pre+ 5 µL HTRFcompounds Activation Buffer. FOR HTRF CERTIFIED READER. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Incubate overnight at RT. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . FOR HTRF CERTIFIED READER For. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. HTRF certified . The donor and acceptor can be used to label a variety of biomolecules, for. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. FOR HTRF CERTIFIED READER. The Bradford assay was successfully performed on the BMG LABTECH microplate reader.